Micropropagation of Saffron / Crocus sativus

Protocol requested for multiplication of Crocus sativus on 22.09.2019.

Saffron is propagated by corms due to its triploidy which leads to sterility. Several protocols describe methods for in vitro multiplication and regeneration of C. sativus, for example:

Microcorm formation using leaf explants (Raja et al. 2006)

  • Medium: Murashige & Skoog (MS) Medium + BA and NAA or 2,4-D (usage of NAA lead to maximum proliferation rate whereas usage of 2,4-D lead to maximum survival rate)

Somatic embryogenesis by usage of embryogenic calli, starting with leaf explants (Raja et al. 2006)

  • Media:
    • MS Medium + BA and 2,4-D for embryogenic calli formation and somatic embryogenesis;
    • MS Medium + GA3 and ABA for germination of matured embryos

Cormlet generation using tissue culture derived shoots (Sharma et al. 2008)

  • Medium: MS Medium + BA


Raja, W., G. Zaffer, and S. A. Wani. “In vitro microcorm formation in saffron (Crocus sativus L.).” II International Symposium on Saffron Biology and Technology 739 . 2006.

Sharma, K. D., et al. “In vitro cormlet development in Crocus sativus.” Biologia Plantarum 52.4 (2008): 709-712.