Hi all,
Does anyone have medium & protocol for Amorphophallus muelleri?
Welcome aboard…
There were several protocols described. Here are two abstracts from publications of an Indonesian research group, unfortunately they used the toxic HgCl2 for explant sterilization in both. Maybe you can find an alternative solution by yourself.
Imelda et al. (2007):
[…] In this research, young shoots which had just appeared from tubers were used as a source of explants. Sterilization of the explants was carried out in 0.05 % HgCl, solution for 20 min, rinsed several times with sterile distilled water and then cultured on Murashige and Skoog (MS) medium containing 0.1-0.2 mg/l Thidiazuron (TDZ), 0.5-1.0 mg/l Benzylaminopurine (BAP) and 0.5-1.0 mg/l Kinetin (KIN) singly or in combination. Acclimatization of plantlets was done on 3 kinds of media namely (A), soil + compost, (B) soil + compost.+ cocopeat, and ( C ) soil + cocopeat. The results showed that the best medium is MS containing 0.2 mg/l TDZ and 0.5 mg/l BAP for in vitro shootbuds induction and proliferation of iles-iles, while MS without plant growth regulators is suitable for shoot growth and root formation and soil + compost + cocopeat for acclimatization of plantlets.
Imelda et al. (2008)
In this investigation, an in vitro method for shoot regeneration of iles-iles from petiole explants was developed. Sterilization of the explants was carried out in 0.05% HgCl2 for 20 min. after dipping in 70% ethanol and Tween 20 solution. Leaf petioles about 1 cm in length were cultured on Murashige & Skoog (MS) medium with a pH of 5.8 containing 30 g sucrose and 2.5 g Gelrite agar. The formation of adventives shoots was induced on MS medium containing 1, 2, and 4 mg/L Benzyl Amino Purine (BAP) either with or without the addition of 0.1, 0.2 and 0.5 mg/L Naphthalene Acetic Acid (NAA). Each treatment was done on 10 explants. All cultures were incubated at 26°C on a 16-h photoperiod with an illumination of 30 μmol m^-2 sec^-1 provided by 40-W cool white inflorescent lights. The highest rate of shoot multiplication averaging 19 shoots per explants was achieved within 3 months on MS medium containing 2 mg/L BAP. However, the best shoot elongation was found on MS medium containing 2 mg/L BAP and 0.2 mg/L NAA.
Another has been described by Prayana et al. in 2017.
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